Large Blue
Large Blue
Why would heating Biuret's solution and a large amount of protein cause a colour change from violet to blue?
A student heats a test tube containing a large amount of protein and Biuret reagent. She notices a colour change from violet to blue. Explain why heating causes a colour change.
Proteins are polypeptides that are composed of amino acids -- each amino acid is connected by a peptide bond.
Biuret's solution changes color (from blue to purple) in the presence of peptide bonds. A reverse reaction would indicate a lose of peptide bonds. Thus, the protein is being denatured.
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